SciELO - Scientific Electronic Library Online

 
vol.62 issue9The changes of muscle FGF-6 expression at different time points after exercise-induced muscle damage author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO

Bookmark

West Indian Medical Journal

Print version ISSN 0043-3144

Abstract

HOU, G et al. Chemokine receptor 7 implications of spleen dendritic cells in multiple-organ dysfunction syndrome in mice. West Indian med. j. [online]. 2013, vol.62, n.9, pp. 787-792. ISSN 0043-3144.

OBJECTIVE: This study aims to explore the chemokine receptor 7 (CCR7) expression of spleen dendritic cells (DCs) and their role in the changes of migration and activity of spleen DCs in multiple-organ dysfunction syndrome (MODS). METHODS: The MODS model of mice was reproduced. The mice were randomly assigned to the following groups: normal, three-hour to six-hour, 24-hour to 48-hour, and 10-day to 12-day postzymosan injection. CD11c and CD205 were analysed by immunohistochemistry; the expressions of CD86 and CCR7 of DCs were studied using flow cytometry analyses. RESULTS: In normal mice, many DCs were found at the margin between the red and white pulp. In the three-hour to six-hour and 24- to 48-hour group, DC effectively upregulated CD86 and CCR7, and they were distributed in T-cell areas. In the 10-day to 12-day group, DCs were distributed at the margin by the immature form. CONCLUSION: The CCR7 expression level of DCs had close correlations with the migration of DCs. Chemokine receptor 7 can be used to evaluate the migration and functional activity of DCs in MODS.

Keywords : Chemokine receptor 7 (CCR7); dendritic cell; multiple organ dysfunction syndrome (MODS); spleen.

        · abstract in Spanish     · text in English     · pdf in English